Summary of the taxa accounting for 85% of the lake littoral benthic macroinvertebrates collected in each of several highly-sampled geographic areas, with taxa grouped by order level or higher in pie charts placed spatially to indicate sampling area. Pie charts correspond to (1) Alaska, (2) Greenland low Arctic, (3) Iceland, and (4) Fennoscandia. State of the Arctic Freshwater Biodiversity Report - Chapter 4 - Page 69 - Figure 4-33

EBSAs (Source: CBD 2016) and marine “areas of heightened ecological and cultural significance” (Source: AMAP/CAFF/SDWG, 2013). In 2013, the Arctic Council identified “Areas of heightened ecological and cultural significance” using the International Maritime Organization criteria for Particularly Sensitive Sea Areas (PSSAs), which are similar to the CBD Ecologically and Biologically Significant Areas (EBSAs) criteria. The term “areas of heightened ecological and cultural significance” comes from Recommendation IIC of the Arctic Council’s 2009 Arctic Marine Shipping Assessment: ARCTIC PROTECTED AREAS - INDICATOR REPORT 2017

Numbers and taxonomic composition of five single-celled eukaryote groups for the regional divisions of the Arctic Marine Areas (pie charts), as well as the number of data sources reviewed across the Arctic (red circles). Total number of taxa is given in parenthesis after each region. Flagellates include: chlorophytes, chrysophytes, cryptophytes, dictyochophytes, euglenids, prasinophytes, prymnesiophytes, raphidophytes, synurales, and xanthophytes, and- for practical purposes though not flagellates - cyanophytes. Heterotrophs include: choanoflagellates, kinetoplastea, incertae sedis. Updated from Poulin et al. (2011). STATE OF THE ARCTIC MARINE BIODIVERSITY REPORT - <a href="https://arcticbiodiversity.is/findings/sea-ice-biota" target="_blank">Chapter 3</a> - Page 39- Figure 3.1.3 From the report draft: "For a pan-Arctic assessment of diversity (here defined as species richness), the first comprehensive assessments of this FEC from a few years ago (Poulin et al. 2011, Daniëls et al. 2013) have been updated for regions, with taxonomic names standardized according to the World Register of Marine Species (www.marinespecies.org). For the analysis of possible interannual trends in the ice algal community, we used a data set from the Central Arctic, the area most consistently and frequently sampled (Melnikov 2002, I. Melnikov, Shirshov Institute, unpubl. data). Multivariate community structure was analysed based on a presence-absence matrix of cores from 1980 to 2013. The analysis is biased by the varying numbers of analysed cores taken per year ranging widely from 1 to 24, ice thickness between 0.6 and 4.2 m, and including both first-year as well as multiyear sea ice. Locations included were in a bounding box within 74.9 to 90.0 °N and 179.9°W to 176.6°E and varied among years."

Temperature and copepod abundance in Zackenberg, northeastern Greenland. Temperature is measured at 80 m for Microcalanus and 5 m for Pseudocalanus (Arendt et al. 2016). STATE OF THE ARCTIC MARINE BIODIVERSITY REPORT - <a href="https://arcticbiodiversity.is/findings/plankton" target="_blank">Chapter 3</a> - Page 76 - Figure 3.2.7

Fish species observations from Traditional Knowledge (TK ) literature, plotted in the approximate geographic location of observed record, with symbol colour indicating the number of fish species recorded and shape indicating the approximate time period of observation. Results are from a systematic literature search of TK sources from Alaska, Canada, Greenland, Fennoscandia, and Russia. State of the Arctic Freshwater Biodiversity Report - Chapter 4 - Page 75- Figure 4-37

Figure 3-4 Effects of permafrost thaw slumping on Arctic rivers, including (upper) a photo of thaw slump outflow entering a stream on the Peel Plateau, Northwest Territories, Canada, and (lower) log10-transformed total suspended solids (TSS) in (1) undisturbed, (2) 1-2 disturbance, and (3) > 2 disturbance stream sites, with letters indicating significant differences in mean TSS among disturbance classifications Plot reproduced from Chin et al. (2016). State of the Arctic Freshwater Biodiversity Report - Chapter3 - Page 21 - Figure 3-4

Results of circumpolar assessment of lake zooplankton, focused just on crustaceans, and indicating (a) the location of crustacean zooplankton stations, underlain by circumpolar ecoregions; (b) ecoregions with many crustacean zooplankton stations, colored on the basis of alpha diversity rarefied to 25 stations; (c) all ecoregions with crustacean zooplankton stations, colored on the basis of alpha diversity rarefied to 10 stations; (d) ecoregions with at least two stations in a hydrobasin, colored on the basis of the dominant component of beta diversity (species turnover, nestedness, approximately equal contribution, or no diversity) when averaged across hydrobasins in each ecoregion. State of the Arctic Freshwater Biodiversity Report - Chapter 4 - Page 58 - Figure 4-25

Estimation of diatom assemblage changes over a period of about 200 years (top versus bottom sediment cores). State of the Arctic Freshwater Biodiversity Report - Chapter 4 - Page 41 - Figure 4-14

Trends in kittiwake colonies 2001-2010, based on linear regression with year as the explanatory variable. Slope of the regression is red = negative trend, blue = positive trend; shaded circle = significant trend (at p<0.05), open circle = non-significant trend. Non-significant deviation from zero could imply a stable population, but in some cases was due to low sample size and low power. Provided with permission from Descamps et al. (in prep). STATE OF THE ARCTIC MARINE BIODIVERSITY REPORT - <a href="https://arcticbiodiversity.is/findings/seabirds" target="_blank">Chapter 3</a> - Page 135 - Figure 3.5.3 This figure is compiled from data from researchers working throughout circumpolar regions, primarily members of the Circumpolar Seabird Group, an EN of CAFF/seabirds. Dr. Sebastien Decamps conducted the analysis and produced the original figure; the full results will be available in an article in prep titled: “Descamps et al. in prep. Circumpolar dynamics of black-legged kittiwakes track large-scale environmental shifts and oceans' warming rate.” [expected submission spring 2016]. Colony population trends were analyzed using a linear regression with the year as explanatory variable. Based on slope of the regression (which cannot be exactly 0) colonies are either Declining (Slope of the regression <0) or Increasing (Slope of the regression >0). (Colonies may have had a negative but not significant slope, and could be stable but for some others, the slope is not significant due to small sample size / low power; thus we cannot say that all colonies with a non- significant slope are stable. The threshold was put at 5% to assess the significance of the trend.

30-year trends in alpha diversity of benthic macroinvertebrates in the stony littoral zones (1 m depth) of two Scandinavian Arctic/alpine lakes: Lake Abiskojaure (upper panel) and Lake Stor-Tjulträsk (lower panel). State of the Arctic Freshwater Biodiversity Report - Chapter 4 - Page 71- Figure 4-35