Abiotic drivers in North America, including (a) long-term average maximum August air temperature, (b) spatial distribution of ice sheets in the last glaciation of the North American Arctic region, and (c) geological setting of bedrock geology underlying North America. Panel (a) source Fick and Hijmans (2017). Panel (b) adapted from: Physical Geology by Steve Earle, freely available at http://open.bccampus.ca. Panel (c) source: Geogratis. State of the Arctic Freshwater Biodiversity Report - Chapter 5 - Page 86 - Figure 5-3

Numbers and taxonomic composition of five single-celled eukaryote groups for the regional divisions of the Arctic Marine Areas (pie charts), as well as the number of data sources reviewed across the Arctic (red circles). Total number of taxa is given in parenthesis after each region. Flagellates include: chlorophytes, chrysophytes, cryptophytes, dictyochophytes, euglenids, prasinophytes, prymnesiophytes, raphidophytes, synurales, and xanthophytes, and- for practical purposes though not flagellates - cyanophytes. Heterotrophs include: choanoflagellates, kinetoplastea, incertae sedis. Updated from Poulin et al. (2011). STATE OF THE ARCTIC MARINE BIODIVERSITY REPORT - <a href="https://arcticbiodiversity.is/findings/sea-ice-biota" target="_blank">Chapter 3</a> - Page 39- Figure 3.1.3 From the report draft: "For a pan-Arctic assessment of diversity (here defined as species richness), the first comprehensive assessments of this FEC from a few years ago (Poulin et al. 2011, Daniëls et al. 2013) have been updated for regions, with taxonomic names standardized according to the World Register of Marine Species (www.marinespecies.org). For the analysis of possible interannual trends in the ice algal community, we used a data set from the Central Arctic, the area most consistently and frequently sampled (Melnikov 2002, I. Melnikov, Shirshov Institute, unpubl. data). Multivariate community structure was analysed based on a presence-absence matrix of cores from 1980 to 2013. The analysis is biased by the varying numbers of analysed cores taken per year ranging widely from 1 to 24, ice thickness between 0.6 and 4.2 m, and including both first-year as well as multiyear sea ice. Locations included were in a bounding box within 74.9 to 90.0 °N and 179.9°W to 176.6°E and varied among years."

Summary of the taxa accounting for 85% of the lake littoral benthic macroinvertebrates collected in each of several highly-sampled geographic areas, with taxa grouped by order level or higher in pie charts placed spatially to indicate sampling area. Pie charts correspond to (1) Alaska, (2) Greenland low Arctic, (3) Iceland, and (4) Fennoscandia. State of the Arctic Freshwater Biodiversity Report - Chapter 4 - Page 69 - Figure 4-33

Results of circumpolar assessment of lake zooplankton, including crustaceans and rotifers, and indicating (a) the location of zooplankton stations, underlain by circumpolar ecoregions; (b) ecoregions with many zooplankton stations, colored on the basis of alpha diversity rarefied to 25 stations; (c) all ecoregions with zooplankton stations, colored on the basis of alpha diversity rarefied to 10 stations; (d) ecoregions with at least two stations in a hydrobasin, colored on the basis of the dominant component of beta diversity (species turnover, nestedness, approximately equal contribution, or no diversity) when averaged across hydrobasins in each ecoregion. State of the Arctic Freshwater Biodiversity Report - Chapter 4- Page 59 - Figure 4-26

Figure 4 -36 Freshwater fish sampling stations (A), ecoregion alpha diversity in each of the sampled ecoregions, as quantified by estimates of species richness from reference texts (Muus and Dahlstrøm 1971, Scott and Crossman 1973, Mecklenburg et al. 2002) and expert knowledge (academic and government scientists and traditional knowledge) (B), and ecoregion beta diversity (C) characterized according to components of beta diversity as either nestedness, turnover, no diversity (none, beta = 0), or similar nestedness and turnover (nestedness ~ turnover) in the circumpolar Arctic. Ecoregions are shown only where sampling stations occur. Fish sampling stations included in this study assessed complete fish assemblages at each location. State of the Arctic Freshwater Biodiversity Report - Chapter 4 - Page 74 - Figure 4-36

Population estimates and trends for Rangifer populations of the migratory tundra, Arctic island, mountain, and forest ecotypes where their circumpolar distribution intersects the CAFF boundary. Population trends (Increasing, Stable, Decreasing, or Unknown) are indicated by shading. Data sources for each population are indicated as footnotes. STATE OF THE ARCTIC TERRESTRIAL BIODIVERSITY REPORT - Chapter 3 - Page 70 - Table 3.4

EBSAs (Source: CBD 2016) and marine “areas of heightened ecological and cultural significance” (Source: AMAP/CAFF/SDWG, 2013). In 2013, the Arctic Council identified “Areas of heightened ecological and cultural significance” using the International Maritime Organization criteria for Particularly Sensitive Sea Areas (PSSAs), which are similar to the CBD Ecologically and Biologically Significant Areas (EBSAs) criteria. The term “areas of heightened ecological and cultural significance” comes from Recommendation IIC of the Arctic Council’s 2009 Arctic Marine Shipping Assessment: ARCTIC PROTECTED AREAS - INDICATOR REPORT 2017

Alpha diversity (rarefied to 10 stations, with error bars indicating standard error) of river benthic macroinvertebrates plotted as a function of the average latitude of stations in each hydrobasin. Hydrobasins are coloured based on country/region State of the Arctic Freshwater Biodiversity Report - Chapter 4 - Page 68 - Figure 4-32

For the background of data production and data interpretation we refer to the PAME report “Modelling Arctic oceanographic connectivity to further develop PAME’s MPA toolbox”. The uploaded data consist of two main types: 1. Connectivity matrices describing the seascape connectivity in the model domain consisting of 40893 model grid cells. The connectivity matrices describe the probability of dispersal between any two selected model grid cells. 2. GIS shape files of dispersal distance (km) from each model grid cell within the model domain.

Appendix 11. Taxa of hetorotrophic protists reported from Foxe Basin, Canada (FB), Disko Bay, W Greenland (DB; Vors 1993), the Greenland Sea (GLS; Ikävalko & Gradinger 1997) and Northern Baffin Bay, Canada (NBB; Lovejoy et al. 2002).